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Myh11-creert2 rosa

WebThe Jackson Laboratory double heterozygous myh11 creert2 rosa26 il11 Double Heterozygous Myh11 Creert2 Rosa26 Il11, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS … Web3 feb. 2024 · TgMyh11 CreERT2; Tcf21 flox/flox;Rosa26Sor tm14(CAGtdTomato)Hze; Apoe −/−: Male (Myh11 CreERt2 transgene is linked to chromosome Y: HFD (0.15% chol, 21% milk fat, Dyets TD 31359001); SD: 8–16 w: scRNA-seq; CITE-seq: Enzymatic digestion …

Split-CreERT2: Temporal Control of DNA Recombination …

Web5 jun. 2024 · Open in a separate window Fig. Cannabiscetin distributor 1 Mouse monoclonal to IHOG Myh11-CreERT2 ROSA eYFP efficiently labeled SMC and a large subset of pericytes in multiple microvascular tissue beds. a Schematic showing crossing of Myh11 … WebMyh11-CreERT2 (but not Itga8-CreERT2) mice exhibited high levels of CreERT2 protein, tamoxifen-independent activity, and an altered transcriptome. Whereas Myh11-CreERT2-mediated knockout of Srf resulted in a lethal intestinal phenotype, loss of Srf with Itga8 … lampada uv h7 https://mimounted.com

Double Heterozygous Myh11 Creert2 Rosa26 Il11 The Jackson …

Web2 dec. 2024 · Purpose: To establish Myh11 as a marker of a subset of corneal endothelial cells (CECs), and to demonstrate the feasibility of restoring the corneal endothelium with Myh11-lineage (Myh11-Lin [+]) adipose-derived stromal cells (ASCs). Methods: … WebTg (Myh11-icre/ERT2)1Soff. Mutation details : The mouse promoter from BAC clone 26630 was used to drive inducible icre expression in smooth muscle cells. An frt-flanked beta-lactamase gene contained within the cre/ESR1 cassette was removed by flp mediated … WebTissue specificity of Myh11 cre ER (T2) activity. 5-week old Myh11 creER (T2)-/+ mTmG-/+ mice were treated with tamoxifen (1 mg,IP) once a day for 5 days. 6 weeks later tissues were harvested... lampada uv hqi

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Category:KLF4-dependent phenotypic modulation of smooth muscle cells …

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Myh11-creert2 rosa

Klf4, Klf2, and Zfp148 activate autophagy-related genes in smooth ...

Web1 jun. 2024 · Myh11-CreERT2 ROSA STOPfl/fl tdTomato mice 6–12 weeks of age were injected intraperitoneally with 1 mg tamoxifen each day for 10 days over two weeks. Tracheal bleomycin administration. Animals were anesthetized with a ketamine/xylazine … Web30 dec. 2024 · Myh11 lineage reporter mouse labels pericytes in the lung microvasculature. The induction of Myh11-CreERT2 ROSA STOPfl/fl tdTomato mice (described in Supplemental Fig. 1A) with tamoxifen induces recombination and expression of …

Myh11-creert2 rosa

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Web29 jul. 2024 · The final genotypes of the SMC lin Tcf21 knockout (SMC lin-KO) mice were: Tg Myh11-CreERT2, Tcf21 ∆SMC/∆SMC, ROSA tdT/+ and ApoE −/ ... WebTg(Ccr2-cre/ERT2)12Kuan - Cardiovascular System Recombinase Activity Detail Allele Information Tissue Information Recombinase Activity References

Web15 dec. 2024 · Myh11-CreER T2-RAD transgene insertion was determined to be on mouse chromosome 2. Myh11-CreER T2-RAD fluorescent reporter mice showed Cre-dependent, tamoxifen-inducible labeling of SMCs equivalent to the widely used Myh11-CreER T2 mice. WebMyh11-CreERT2 ROSA eYFP efficiently labeled SMC and a large subset of pericytes in multiple microvascular tissue beds. a Schematic showing crossing of Myh11-CreERT2 ROSA floxed STOP eYFP mice...

WebNational Center for Biotechnology Information Web28 aug. 2024 · The Myh11-CreER T2 mouse line (Cre +) has gained increasing application because of its high lineage specificity relative to other Cre drivers targeting smooth muscle cells (SMCs).This Cre allele, however, was initially inserted into the Y chromosome (X/Y …

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Web17 sep. 2024 · Myh11 CreERT2; Braf LSL‐V600E/+ mice developed not only ICC hyperplasia but also GIST and in the absence of Trp53 disruption. In addition to providing a simpler model for mutant Braf GIST, these results provide conclusive evidence for … jessica lm juba lamWeb17 nov. 2024 · The Myh11-CreERT2 ROSA STOPfl/fl tdTomato reporter mouse requires tamoxifen for reporter induction and does not alter lung histology. (A) Schematic of the Myh11-CreERT2 ROSA STOPfl/fl tdTomato lineage reporter mouse (B) Representative … lampada uv grandeWeb10 jan. 2024 · Shankman et al. demonstrated that the contribution of SMCs within atherosclerotic plaques has been extremely neglected and that the transformation of the SMC phenotype mediated by KLF4 is crucial in lesion pathogenesis utilizing a Myh11-CreERT2 ROSA floxed STOP eYFP ApoE −/− mouse model . jessica lm instagramWebFigure Lengend Snippet: The Myh11-CreERT2 ROSA STOPfl/fl tdTomato reporter mouse labels pericytes in the lung capillary bed. (A) Representative immunofluorescence (IF) micrographs of lung sections stained for tdTomato (red, endogenous fluorophore), αSMA … lampada uv germicida 15w t8WebFigure Legend Snippet: (A,B) c-Myc in situ hybridization on ctrl (A) and Myh11-cre;c-Myc f/f (B) lungs 7 days after naphthalene injury, showing that c-Myc is expressed by airway smooth muscle cells after airway epithelial injury and the absence of c-Myc expression in … lampada uv gemmologiaWebThis assay will NOT distinguish hemizygous from homozygous transgenic animals. The genotyping protocol (s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends … lampada uv ledWebROSA mT/mG mice carry a single copy of the gene cassette comprised of cytomegalovirus enhancer and b-actin chimeric promoter (pCAG) and a floxed membrane localized Tomato followed by a membrane... jessica lm juba lami mp3 download fakaza